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The following is a sample recipe for TBST: 20 mM Tris; 150 mM NaCl; 0.1% Tween 20; Adjust pH with HCl to pH 7.4–7.6 The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water. [1]
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
Edwin Southern first created the Southern blot, [1] an analytical technique used to detect DNA. The technique involves using gel electrophoresis, an important analytical method that involves the use of an electric field and the subsequent migration of charged DNA, RNA or proteins through that electric field based on size and charge. [2]
Its name is an almost-acronym of bovine lacto transfer technique optimizer. It constitutes an inexpensive source of nonspecific protein (milk casein) which blocks protein binding sites in a variety of experimental paradigms, notably Southern blots, Western blots, and ELISA. Its use was first reported in 1984 by Johnson and Elder's lab at Scripps.
Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.
A blocking antibody is an antibody that does not have a reaction when combined with an antigen, but prevents other antibodies from combining with that antigen. [1] This function of blocking antibodies has had a variety of clinical and experimental uses. The term can also be used for inhibiting antibody, prozone phenomenon and, agglutination ...
This technique relies upon current and a transfer buffer solution to drive proteins or nucleic acids onto a membrane. Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up.
Tris-buffered saline (TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of Tris has a pK a of 8.07 at 25 °C. The pK a declines approximately 0.03 units per degree Celsius rise in ...