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Subterminal endospores are those between these two extremes, usually seen far enough towards the poles but close enough to the center so as not to be considered either terminal or central. Lateral endospores are seen occasionally. Examples of bacteria having terminal endospores include Clostridium tetani, the pathogen that causes the disease ...
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1] Within bacteria, endospores are protective structures used to survive extreme conditions, including high temperatures making them highly resistant to chemicals. [ 2 ]
Detects the presence of endospores in six genera of bacteria Endospores: Green Vegetative cells: Red 6 Capsule A: Hiss method (Positive technique) B: Manevals's technique (Negative) Smear stained with Hiss stain following treatment with copper sulphate Bacterial suspension smeared along with Congo red and the Maneval's stain is applied
Clostridium sporogenes is a species of Gram-positive bacteria that belongs to the genus Clostridium. Like other strains of Clostridium, it is an anaerobic, rod-shaped bacterium that produces oval, subterminal endospores [2] and is commonly found in soil. Unlike Clostridium botulinum, it does not produce the botulinum neurotoxins.
Clostridium septicum is a resident bacterium of the human microflora, however it can be found in almost any anoxic habitat in which exists organic compounds. [4] Under unfavorable conditions, C. septicum forms endospores allowing it to survive under harsh conditions such as extreme temperature, dry land, and nutrient-deficient habitats.
A stained preparation of Bacillus subtilis showing endospores as green and the vegetative cell as red. The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. [1]
Some bacteria also produce endospores with an exosporium, of which the most commonly studied are Bacillus species, particularly Bacillus cereus and the anthrax-causing bacterium Bacillus anthracis. The exosporium is the portion of the spore that interacts with the environment or host organism, and may contain spore antigens. [1]
[2] [3] [4] B. cereus bacteria may be aerobes or facultative anaerobes, and like other members of the genus Bacillus, can produce protective endospores. They have a wide range of virulence factors, including phospholipase C, cereulide, sphingomyelinase, metalloproteases, and cytotoxin K, many of which are regulated via quorum sensing.