Ad
related to: housekeeping genes for western blot technology examples- Western Blotting Handbook
New, updated technical guide.
Download your copy now.
- Explore our Brochure
See what iBright has to offer.
Download, print, and share.
- Examine Features
Discover our suite of features.
Learn about the benefits of iBright
- 21 CFR part 11 Support
Learn about compliance support.
Security, auditing & e-signatures.
- iBright Imaging Systems
Find which model is right for you.
iBright CL750, CL1500, or FL1500.
- Watch Videos
View our demo videos.
Get a glimpse of the experience.
- Western Blotting Handbook
Search results
Results From The WOW.Com Content Network
Housekeeping genes and proteins, including β-Actin, GAPDH, HPRT1, and RPLP1, are often used as internal controls in western blots because they are thought to be expressed constitutively, at the same levels, across experiments.
Housekeeping genes account for majority of the active genes in the genome, and their expression is obviously vital to survival. The housekeeping gene expression levels are fine-tuned to meet the metabolic requirements in various tissues.
A housekeeping gene is a gene that is required to maintain basic cellular function and so is typically expressed in all cell types of an organism. Examples include actin , GAPDH and ubiquitin . Some housekeeping genes are transcribed at a relatively constant rate and these genes can be used as a reference point in experiments to measure the ...
Although the terms "housekeeping gene" and "reference gene" are used somewhat interchangeably, caution must be used in selecting genes for reference purposes. Suitable reference genes must be stably expressed in the specific experimental condition or tissue of interest. [ 1 ]
Actin beta is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa. [citation needed]
Immunoproteomics example experiment involving western blot analysis. Immunoproteomics is the study of large sets of proteins involved in the immune response. Examples of common applications of immunoproteomics include: The isolation and mass spectrometric identification of MHC (major histocompatibility complex) binding peptides
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
Calnexin (CNX) is a 67kDa integral protein (that appears variously as a 90kDa, 80kDa, or 75kDa band on western blotting depending on the source of the antibody) of the endoplasmic reticulum (ER). It consists of a large (50 kDa) N-terminal calcium-binding lumenal domain, a single transmembrane helix and a short (90 residues), acidic cytoplasmic ...
Ad
related to: housekeeping genes for western blot technology examples