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  2. Cloning - Wikipedia

    en.wikipedia.org/wiki/Cloning

    Two commonly discussed types of theoretical human cloning are therapeutic cloning and reproductive cloning. Therapeutic cloning would involve cloning cells from a human for use in medicine and transplants, and is an active area of research, but is not in medical practice anywhere in the world, as of 2024.

  3. Human cloning - Wikipedia

    en.wikipedia.org/wiki/Human_cloning

    Therapeutic cloning would involve cloning cells from a human for use in medicine and transplants. It is an active area of research, and is in medical practice over the world. Two common methods of therapeutic cloning that are being researched are somatic-cell nuclear transfer and (more recently) pluripotent stem cell induction .

  4. In vitro recombination - Wikipedia

    en.wikipedia.org/wiki/In_vitro_recombination

    There are many types of cloning vectors such as plasmids and phages. In order to carry out recombination between vector and the foreign DNA, it is necessary the vector and DNA to be cloned by digestion, ligase the foreign DNA into the vector with the enzyme DNA ligase. And DNA is inserted by introducing the DNA into bacteria cells by transformation

  5. Somatic cell nuclear transfer - Wikipedia

    en.wikipedia.org/wiki/Somatic_cell_nuclear_transfer

    "Therapeutic cloning" refers to the potential use of SCNT in regenerative medicine; this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESCs) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are.

  6. De-extinction - Wikipedia

    en.wikipedia.org/wiki/De-extinction

    There are several ways to carry out the process of de-extinction. Cloning is the most widely proposed method, although genome editing and selective breeding have also been considered. Similar techniques have been applied to certain endangered species, in hopes to boost their genetic diversity. The only method of the three that would provide an ...

  7. Artificial gene synthesis - Wikipedia

    en.wikipedia.org/wiki/Artificial_gene_synthesis

    Because Gateway cloning is a proprietary technology, all Gateway reactions must be carried out with the Gateway kit that is provided by the manufacturer. The reaction can be summarised into two steps.

  8. Molecular cloning - Wikipedia

    en.wikipedia.org/wiki/Molecular_cloning

    Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...

  9. Clone (cell biology) - Wikipedia

    en.wikipedia.org/wiki/Clone_(cell_biology)

    Most other cells cannot divide indefinitely as after a few cycles of cell division the cells stop expressing an enzyme telomerase.The genetic material, in the form of deoxyribonucleic acid (DNA), continues to shorten with each cell division, and cells eventually stop dividing when they sense that their DNA is critically shortened.