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Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
In 1978, Still and coworkers published a highly influential paper reporting a purification technique known as flash column chromatography. [1] Prior to this report, column chromatography using silica gel as a stationary phase had already been established as a valuable method for the separation and purification of organic compounds. However ...
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed ...
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In industrial continuous fractionating columns, N t is determined by starting at either the top or bottom of the column and calculating material balances, heat balances and equilibrium flash vaporizations for each of the succession of equilibrium stages until the desired end product composition is achieved.
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