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Fluorescein is a dye which is taken up by damaged cornea such that the area appears green under cobalt blue light. [3] There is also a version that comes premixed with lidocaine. [4] [8] Fluorescein was first made in 1871. [9] It is on the World Health Organization's List of Essential Medicines. [10]
Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water).
Slit lamp bio microscope: used for examining the anteriorly placed structures the eye; video link: Charts for vision - •Distant vision: to determine visual acuity of distant vision ••Snellen's distant vision chart-do-; for those who can read in English ••Regional language charts-do-; for those who can read in their local language ...
The fluorescein is administered intravenously in intravenous fluorescein angiography (IVFA) and orally in oral fluorescein angiography (OFA). The test is a dye tracing method. The fluorescein dye also reappears in the patient urine, causing the urine to appear darker, and sometimes orange. [2] It can also cause discolouration of the saliva.
Rose bengal was originally prepared in 1882 by Swiss chemist Robert Ghnem, as an analogue of fluorescein. [4] Rudolf Nietzki at the University of Basel identified the principal constituents of rose bengal as iodine derivatives of di- and tetra-chlorofluorescein. [5] The compound was originally used as a wool dye. [6]
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [ 3 ] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.