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Plymouth Routines In Multivariate Ecological Research (PRIMER) is a statistical package that is a collection of specialist univariate, multivariate, and graphical routines for analyzing species sampling data for community ecology. [1]
Plotly – plotting library and styling interface for analyzing data and creating browser-based graphs. Available for R, Python, MATLAB, Julia, and Perl; Primer-E Primer – environmental and ecological specific; PV-WAVE – programming language comprehensive data analysis and visualization with IMSL statistical package
For assays of agonist/activation type, the control (c) data (, ) in the equation are substituted with the positive control (p) data (, ) which represent maximal activated signal; for assays of antagonist/inhibition type, the control (c) data (, ) in the equation are substituted with the negative control (n) data (, ) which represent minimal signal.
Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple targets with only a single primer pair. [1] It detects copy number changes at the molecular level, and software programs are used for analysis.
Such primers will anneal only to sequences that are methylated, and thus containing 5-methylcytosines that are resistant to conversion by bisulfite. In alternative fashion, unmethylated-specific primers can be used. This alternative method of methylation analysis also uses bisulfite-treated DNA but avoids the need to sequence the area of ...
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
The Ribosomal Database Project (RDP) was a curated database that offers ribosome data along with related programs and services. The offerings included phylogenetically ordered alignments of ribosomal RNA (rRNA) sequences, derived phylogenetic trees, rRNA secondary structure diagrams and various software packages for handling, analyzing and ...
Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the mRNA.