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  2. Internal transcribed spacer - Wikipedia

    en.wikipedia.org/wiki/Internal_transcribed_spacer

    In eukaryotes, genes encoding ribosomal RNA and spacers occur in tandem repeats that are thousands of copies long, each separated by regions of non-transcribed DNA termed intergenic spacer (IGS) or non-transcribed spacer (NTS). Each eukaryotic ribosomal cluster contains the 5' external transcribed spacer (5' ETS), the 18S rRNA gene, the ITS1 ...

  3. Spacer DNA - Wikipedia

    en.wikipedia.org/wiki/Spacer_DNA

    Spacer DNA is a region of non-coding DNA between genes. [1] [2] The terms intergenic spacer (IGS) or non-transcribed spacer (NTS) are used particularly for the spacer DNA between the many tandemly repeated copies of the ribosomal RNA genes. [3] In bacteria, spacer DNA sequences are only a few nucleotides long.

  4. Intergenic region - Wikipedia

    en.wikipedia.org/wiki/Intergenic_region

    In humans, intergenic regions comprise about 50% of the genome, whereas this number is much less in bacteria (15%) and yeast (30%). [4] As with most other non-coding DNA, the GC-content of intergenic regions vary considerably among species. For example in Plasmodium falciparum, many intergenic regions have an AT content of 90%. [5]

  5. Ribosomal intergenic spacer analysis - Wikipedia

    en.wikipedia.org/wiki/Ribosomal_intergenic...

    RISA involves PCR amplification of a region of the rRNA gene operon between the small and large subunits called the intergenic spacer region ISR. [2] By using oligonucleotide primers targeted to conserved regions in the 16S and 23S genes, RISA fragments can be generated from most of the dominant bacteria in an environmental sample.

  6. Non-coding RNA - Wikipedia

    en.wikipedia.org/wiki/Non-coding_RNA

    [9] [10] It also has been suggested to simply use the term RNA, since the distinction from a protein coding RNA (messenger RNA) is already given by the qualifier mRNA. [100] This eliminates the ambiguity when addressing a gene "encoding a non-coding" RNA. Besides, there may be a number of ncRNAs that are misannoted in published literature and ...

  7. Eukaryotic transcription - Wikipedia

    en.wikipedia.org/wiki/Eukaryotic_transcription

    A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.

  8. Ribosomal DNA - Wikipedia

    en.wikipedia.org/wiki/Ribosomal_DNA

    The rDNA gene cluster of eukaryotes consists of the genes for the 18S, 5.8S and 28S rRNA, separated by the two ITS-1 and ITS-2 spacers. The active genome of eukaryotes contains several hundred copies of the rDNA transcriptional unit as tandem repeats, they are organized in nucleolus organizer regions (NORs), [4] which in turn can be present at ...

  9. Non-coding DNA - Wikipedia

    en.wikipedia.org/wiki/Non-coding_DNA

    In eukaryotes, the bases of the loops are called scaffold attachment regions (SARs) and they consist of stretches of DNA that bind an RNA/protein complex to stabilize the loop. There are about 100,000 loops in the human genome and each SAR consists of about 100 bp of DNA, so the total amount of DNA devoted to SARs accounts for about 0.3% of the ...