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The enzyme alkaline phosphatase (ALP, alkaline phenyl phosphatase, also abbreviated PhoA) is a phosphatase with the physiological role of dephosphorylating compounds. The enzyme is found across a multitude of organisms, prokaryotes and eukaryotes alike, with the same general function, but in different structural forms suitable to the environment they function in. Alkaline phosphatase is found ...
Chemistry stencils that used to be used for drawing equipment in lab notebooks. A laboratory notebook ( colloq. lab notebook or lab book ) is a primary record of research . Researchers use a lab notebook to document their hypotheses , experiments and initial analysis or interpretation of these experiments.
The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is based on the reaction of Cu +, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction).
Specific activity gives a measurement of enzyme purity in the mixture. It is the micro moles of product formed by an enzyme in a given amount of time (minutes) under given conditions per milligram of total proteins. Specific activity is equal to the rate of reaction multiplied by the volume of reaction divided by the mass of total protein.
ALP activity is significantly increased in the third trimester of pregnancy. [11] This is due to increased synthesis from the placenta as well as increased synthesis in the liver induced by large amounts of estrogens. [11] [12] [13] Levels in the third trimester can be as much as 2-fold greater than in non-pregnant women. [11]
In the initially published VCC experiments [1] only the internal 60 wells of the plate were used, since evaporation changed the volume of the edge wells during the 12-hour incubation. However, all 96 wells of the 96-well plate can be used for the experiment as long as the edge of the plate is wrapped with a piece of Parafilm M six squares long ...
This property can be used to determine the activity of various phosphatases including alkaline phosphatase (AP) and protein tyrosine phosphatase (PTP). [ 3 ] PNPP is classified as a chromogenic substrate because of its ability to transform from a colorless compound to a colored compound through a biological mechanism, dephosphorylation. [ 4 ]
248 11648 Ensembl ENSG00000163295 ENSMUSG00000036500 UniProt P09923 P24822 RefSeq (mRNA) NM_001631 NM_007432 RefSeq (protein) NP_001622 n/a Location (UCSC) Chr 2: 232.46 – 232.46 Mb Chr 1: 87.05 – 87.06 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase that in humans is encoded by the ALPI gene ...