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This article contains a list of the most studied restriction enzymes whose names start with Ba to Bc inclusive. It contains approximately 120 enzymes. The following information is given: Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature [1] [2], and bibliographical references.
This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of Restriction Enzyme : Accepted name of the molecule , according to the internationally adopted nomenclature , [ 1 ] [ 2 ] and bibliographical references .
A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria.One special kind of restriction enzymes is the class of "homing endonucleases", these being present in all three domains of life, although their function seems to be very different from one domain to another.
The restriction modification system (RM system) is found in bacteria and archaea, and provides a defense against foreign DNA, such as that borne by bacteriophages.. Bacteria have restriction enzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific points into fragments, which are then degraded further by other endonucleases.
A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. [1] [2] [3] Restriction enzymes are one class of the broader endonuclease group of enzymes.
Type III site-specific deoxyribonuclease (EC 3.1.21.5, type III restriction enzyme, restriction-modification system) is an enzyme. [1] This enzyme catalyses the following chemical reaction. Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes . In molecular biology , restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA.
The number of copies of the gene is then amplified using polymerase chain reaction (PCR). Simultaneously, the same restriction enzymes are used to digest (cut) the destination. The idea behind using the same restriction enzymes is to create complementary sticky ends, which will facilitate ligation later on.