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  2. Western blot - Wikipedia

    en.wikipedia.org/wiki/Western_blot

    The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. Proteins from known HIV-infected cells are separated and blotted on a membrane as above. Then, the serum to be tested is applied in the primary antibody incubation step; free antibody is washed away, and a secondary anti-human antibody linked ...

  3. Diagnosis of HIV/AIDS - Wikipedia

    en.wikipedia.org/wiki/Diagnosis_of_HIV/AIDS

    In a generally healthy low-risk population, indeterminate results on western blot occur on the order of 1 in 5,000 patients. [15] However, for those individuals who have had high-risk exposures to individuals where HIV-2 is most prevalent, Western Africa, an inconclusive western blot test may prove infection with HIV-2. [16]

  4. Monoclonal antibody - Wikipedia

    en.wikipedia.org/wiki/Monoclonal_antibody

    Proteins can be detected using the Western blot and immuno dot blot tests. In immunohistochemistry , monoclonal antibodies can be used to detect antigens in fixed tissue sections, and similarly, immunofluorescence can be used to detect a substance in either frozen tissue section or live cells.

  5. Envelope glycoprotein GP120 - Wikipedia

    en.wikipedia.org/wiki/Envelope_glycoprotein_GP120

    Since CD4 receptor binding is the most obvious step in HIV infection, gp120 was among the first targets of HIV vaccine research. Efforts to develop HIV vaccines targeting gp120, however, have been hampered by the chemical and structural properties of gp120, which make it difficult for antibodies to bind to it. gp120 can also easily be shed from the surface of the virus and captured by T cells ...

  6. Viral load monitoring for HIV - Wikipedia

    en.wikipedia.org/wiki/Viral_load_monitoring_for_HIV

    HIV is a retrovirus, an RNA virus that enters a host cell and uses the host DNA replication machinery and the enzyme reverse transcriptase to produce DNA from the viral RNA genome. HIV also produces an integrase enzyme which is used to integrate the newly produced viral DNA into the host's DNA. The virus is then replicated every time the host ...

  7. SDS-PAGE - Wikipedia

    en.wikipedia.org/wiki/SDS-PAGE

    In the HIV test, HIV proteins are separated by SDS-PAGE and subsequently detected by Western Blot with HIV-specific antibodies of the patient, if they are present in his blood serum. SDS-PAGE for proteinuria evaluates the levels of various serum proteins in the urine, e.g. Albumin , Alpha-2-macroglobulin and IgG .

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