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A rare-cutter enzyme is a restriction enzyme with a recognition sequence which occurs only rarely in a genome. An example is NotI, which cuts after the first GC of a 5'-GCGGCCGC-3' sequence; restriction enzymes with seven and eight base pair recognition sequences are often also called rare-cutter enzymes (six bp recognition sequences are much more common).
A genome sequence is the complete list of the nucleotides (A, C, G, and T for DNA genomes) that make up all the chromosomes of an individual or a species. Within a species, the vast majority of nucleotides are identical between individuals, but sequencing multiple individuals is necessary to understand the genetic diversity.
Genomics is an interdisciplinary field of molecular biology focusing on the structure, function, evolution, mapping, and editing of genomes.A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dimensional structural configuration.
While there are significant differences among the genomes of human individuals (on the order of 0.1% due to single-nucleotide variants [9] and 0.6% when considering indels), [10] these are considerably smaller than the differences between humans and their closest living relatives, the bonobos and chimpanzees (~1.1% fixed single-nucleotide ...
In comparative genomics, synteny is the preserved order of genes on chromosomes of related species indicating their descent from a common ancestor.Synteny provides a framework in which the conservation of homologous genes and gene order is identified between genomes of different species. [9]
Saccharomyces cerevisiae was the first eukaryotic organism to have its complete genome sequence determined.. This list of "sequenced" eukaryotic genomes contains all the eukaryotes known to have publicly available complete nuclear and organelle genome sequences that have been sequenced, assembled, annotated and published; draft genomes are not included, nor are organelle-only sequences.
The term 'pangenome' was defined with its current meaning by Tettelin et al. in 2005; [2] it derives 'pan' from the Greek word παν, meaning 'whole' or 'everything', while the genome is a commonly used term to describe an organism's complete genetic material.
Chloroplast genomes in photosynthetic organisms are normally 120–200 kb [30] encoding 20–200 proteins [25] and mitochondrial genomes in humans are approximately 16 kb and encode 37 genes, 13 of which are proteins. [31]