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where Δν̃ is the Raman shift expressed in wavenumber, λ 0 is the excitation wavelength, and λ 1 is the Raman spectrum wavelength. Most commonly, the unit chosen for expressing wavenumber in Raman spectra is inverse centimeters (cm −1). Since wavelength is often expressed in units of nanometers (nm), the formula above can scale for this ...
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Stimulated Raman spectroscopy, also referred to as stimulated Raman scattering (SRS), is a form of spectroscopy employed in physics, chemistry, biology, and other fields. . The basic mechanism resembles that of spontaneous Raman spectroscopy: a pump photon, of the angular frequency , which is scattered by a molecule has some small probability of inducing some vibrational (or rotational ...
The spectrum of intensity differences recorded over a range of wavenumbers reveals information about chiral centres in the sample molecule. Raman optical activity can be observed in a number of forms, depending on the polarization of the incident and the scattered light.
A Fermi resonance is the shifting of the energies and intensities of absorption bands in an infrared or Raman spectrum. It is a consequence of quantum-mechanical wavefunction mixing. [1] The phenomenon was first explained by the Italian physicist Enrico Fermi. [2]
By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be measured. Sampling is non-destructive and water, media, and buffers typically do not interfere with the analysis. Consequently, in vivo time- and space-resolved Raman spectroscopy is suitable to examine proteins, cells and ...