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Gurdon's experiments captured the attention of the scientific community as it altered the notion of development and the tools and techniques he developed for nuclear transfer are still used today. The term clone [ 26 ] (from the ancient Greek word κλών (klōn, "twig")) had already been in use since the beginning of the 20th century in ...
In particular, the research of Sir John Gurdon in 1958 entailed the cloning of Xenopus laevis utilizing the principles of SCNT. [5] In short, the experiment consisted of inducing a female specimen to ovulate, at which point her eggs were harvested. From here, the egg was enucleated using ultra-violet irradiation to disable the egg's pronucleus.
Experiment: Gurdon used a technique known as nuclear transfer to replace the killed-off nucleus of a frog (Xenopus) egg with a nucleus from a mature cell (intestinal epithelial). The tadpoles resulting from these eggs did not survive long (past the gastrulation stage), however, further transformation of the nuclei from these Xenopus eggs to a ...
Little was known about the function of the nucleolus until 1964, when a study [10] of nucleoli by John Gurdon and Donald Brown in the African clawed frog Xenopus laevis generated increased interest in its function and detailed structure. They found that 25% of the frog eggs had no nucleolus, and that such eggs were not capable of life.
1958 – John Gurdon used nuclear transplantation to clone an African Clawed Frog; first cloning of a vertebrate using a nucleus from a fully differentiated adult cell. 1958 – Matthew Stanley Meselson and Franklin W. Stahl proved that DNA replication is semiconservative in the Meselson-Stahl experiment
A last-minute glitch surfaced on Thursday in the details of the Gaza ceasefire-for-hostages deal and U.S. envoys are working to resolve it, a U.S. official said. Working on the issue is President ...
In 1962, John B. Gurdon demonstrated that the nucleus from a differentiated frog intestinal epithelial cell can generate a fully functional tadpole via transplantation to an enucleated egg. Gurdon used somatic cell nuclear transfer (SCNT) as a method to understand reprogramming and how cells change in specialization. He concluded that ...
Elizabeth Loftus' and John C. Palmer's car crash experiment shows that leading questions can produce false memories (1974) Benjamin Libet's experiment on free will shows that a readiness potential appears before the notion of doing the task enters conscious experience, sparking debate about the illusory nature of free will yet again. (1983)