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IPC – Isoelectric Point Calculator — calculate protein isoelectric point using over 15 methods; prot pi – protein isoelectric point — an online program for calculating pI of proteins (include multiple subunits and posttranslational modifications)
The pzc is the same as the isoelectric point (iep) if there is no adsorption of other ions than the potential determining H + /OH − at the surface [clarification needed]. [8] This is often the case for pure ("pristine surface") oxides in suspension in water. In the presence of specific adsorption, pzc and isoelectric point generally have ...
The iso-electric point is the pH value at which the zeta potential is approximately zero. At a pH near the iso-electric point (± 2 pH units), colloids are usually unstable; the particles tend to coagulate or flocculate. Such titrations use acids or bases as titration reagents. Tables of iso-electric points for different materials are available ...
The equation for determining the isoelectric point is as follows: p I = p K a + p K b 2 {\displaystyle pI={{pKa}+{pKb} \over 2}} In the papermaking process, the pigment dispersions are generally kept at a pH above 8.0.
It is different from the isoelectric point (pI) in that pI is the pH value at which the net charge of the molecule, including bound ions is zero. Whereas the isoionic point is at net charge zero in a deionized solution. Thus, the isoelectric and isoionic points are equal when the concentration of charged species is zero.
Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge; Isoelectric focusing, a technique for separating different molecules by differences in their isoelectric point; Isoelectric line representing the absence of electrical activity on an electrocardiogram
[72] [73] [74] The isoelectric point of a given molecule is a function of its pK values, so different molecules have different isoelectric points. This permits a technique called isoelectric focusing, [75] which is used for separation of proteins by 2-D gel polyacrylamide gel electrophoresis. Buffer solutions also play a key role in analytical ...
Upon the application of pressure on a test solution, liquid starts to flow and to generate an electric potential. This streaming potential is related to the pressure gradient between the ends of either a single flow channel (for samples with a flat surface) or the porous plug (for fibers and granular media) to calculate the surface zeta potential.