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High-level resistance is conferred by the cat-gene; [16] this gene codes for an enzyme called chloramphenicol acetyltransferase, which inactivates chloramphenicol by covalently linking one or two acetyl groups, derived from acetyl-S-coenzyme A, to the hydroxyl groups on the chloramphenicol molecule. The acetylation prevents chloramphenicol from ...
Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) [1] that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. [2] This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes. A ...
This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. An example of a selectable marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, which confers resistance to the antibiotic chloramphenicol. [11]
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
pBR322 is 4361 base pairs in length [1] and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (Amp R) protein, and the gene tetA encoding the tetracycline resistance (Tet R) protein. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number.
PFF1 consists of an origin of replication, oriV, an origin of transfer, oriT, a gene coding for plasmid replication proteins, trfA, and two antibiotic resistance genes, bla and cat, which confer resistance to Ampicillin and Chloramphenicol, respectively. Minimal plasmids such as PFF1 are useful for studying the basic mechanisms of plasmid ...
The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. [ 1 ] [ 2 ] In E. coli , the P BAD promoter is adjacent to the P C promoter (systematically araCp ), which transcribes the araC gene in the opposite direction.
Translational block to expression of the E. coli Tn9-derived chloramphenicol-resistance gene in Bacillus subtilis Proc. Natl. Acad. Sci. USA. 79:5886–5890. Karrer, E.E. and Rodriguez, R.L. 1992. Metabolic regulation of rice alpha-amylase and sucrose synthase genes in planta.