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Heat is applied by baking in a dry heat oven that is designed specifically for the depyrogenation process. Although endotoxins are relatively thermally stable, sufficient heating (250 °C for 30 min) results in a 3-log reduction of endotoxin levels. Due to the high temperature levels, this method is also not suitable when purifying proteins.
Z-value is a term used in microbial thermal death time calculations. It is the number of degrees the temperature has to be increased to achieve a tenfold (i.e. 1 log 10) reduction in the D-value. The D-value of an organism is the time required in a given medium, at a given temperature, for a ten-fold reduction in the number of organisms.
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...
The main feature of thermodynamic diagrams is the equivalence between the area in the diagram and energy. When air changes pressure and temperature during a process and prescribes a closed curve within the diagram the area enclosed by this curve is proportional to the energy which has been gained or released by the air.
Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity. The temperature at which 50% of DNA is denatured is known as the melting temperature. Measurement ...
The principle of time-temperature superposition requires the assumption of thermorheologically simple behavior (all curves have the same characteristic time variation law with temperature). From an initial spectral window [ ω 1 , ω 2 ] and a series of isotherms in this window, we can calculate the master curves of a material which extends ...
Lawson calculated the fusion rate by assuming that the fusion reactor contains a hot plasma cloud which has a Gaussian curve of individual particle energies, a Maxwell–Boltzmann distribution characterized by the plasma's temperature. Based on that assumption, he estimated the first term, the fusion energy being produced, using the volumetric ...
The system does not require solvatochromic dyes, reducing the risk of interferences. The protein samples are simply mixed with the test conditions in a 96-well plate and subjected to a melt-curve protocol using a real-time thermal cycler. The data are obtained within 1–2 h and include unique quality control measures through the GFP signal.