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  2. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    The pharmaceutical industry also regularly employs RP-HPLC to qualify drugs before their release. [citation needed] RP-HPLC operates on the principle of hydrophobic interactions, which originates from the high symmetry in the dipolar water structure and plays the most important role in all processes in life science.

  3. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

  4. Aqueous normal-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Aqueous_normal-phase...

    Aqueous normal-phase chromatography (ANP) [1] is a chromatographic technique that involves the mobile phase compositions and polarities between reversed-phase chromatography (RP) and normal-phase chromatography (NP), while the stationary phases are polar.

  5. Monolithic HPLC column - Wikipedia

    en.wikipedia.org/wiki/Monolithic_HPLC_column

    A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.

  6. Play-by-post role-playing game - Wikipedia

    en.wikipedia.org/wiki/Play-by-post_role-playing_game

    Discord has become a major medium for play-by-chat games due to its rapidly-increasing popularity and its user-friendly administrative features that allow users to create private chat rooms (known on the site as "servers") in very little time. Discord servers listing roleplay servers have also become popular and many have thousands of members.

  7. Hydrophilic interaction chromatography - Wikipedia

    en.wikipedia.org/wiki/Hydrophilic_interaction...

    Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.

  8. Fast protein liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/Fast_protein_liquid...

    Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).

  9. Liquid chromatography–mass spectrometry - Wikipedia

    en.wikipedia.org/wiki/Liquid_chromatography...

    The interface between a liquid phase technique (HPLC) with a continuously flowing eluate, and a gas phase technique carried out in a vacuum was difficult for a long time. The advent of electrospray ionization changed this. Currently, the most common LC–MS interfaces are electrospray ionization (ESI), atmospheric pressure chemical ionization ...