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Intracytoplasmic sperm injection (ICSI / ˈ ɪ k s i / IK-see) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg. This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus.
Intracytoplasmic sperm injection (ICSI) Intracytoplasmic sperm injection (ICSI) is beneficial in the case of male factor infertility where sperm counts are very low or failed fertilization occurred with previous IVF attempt(s). The ICSI procedure involves a single sperm carefully injected into the center of an egg using a microneedle.
Introduced in 2001, the intracytoplasmic morphologically selected sperm injection (IMSI) procedure changed the perception of how a spermatozoon suitable for fertilization (via injection into the egg) should appear. A very high powered lens is used to see the sperm in greater detail during the selection process.
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The presence of abnormally-shaped sperm can negatively affect fertility by preventing transport through the cervix and/or preventing sperm from adhering to the ovum. Achieving a pregnancy may be difficult. [3] In testing for teratozoospermia, sperm are collected, stained and analyzed under a microscope to detect abnormalities.
The main technique used is intracytoplasmic sperm injection (ICSI) where fertilisation is achieved by a single sperm being injected into the egg. [11] Some studies have shown it is possible for a viable embryo to be created with this technique alone, [ 6 ] however others have found it necessary to also use calcium ionophore treatment for ...
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