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Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA ) or plasmid DNA into in vitro cell cultures by lipofection . [ 1 ]
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
A difference engine is an automatic mechanical calculator designed to tabulate polynomial functions. It was designed in the 1820s, and was first created by Charles Babbage . The name difference engine is derived from the method of finite differences , a way to interpolate or tabulate functions by using a small set of polynomial co-efficients.
In thermal engineering, the logarithmic mean temperature difference (LMTD) is used to determine the temperature driving force for heat transfer in flow systems, most notably in heat exchangers. The LMTD is a logarithmic average of the temperature difference between the hot and cold feeds at each end of the double pipe exchanger.
For quality control, one index for the quality of an HTS assay is the magnitude of difference between a positive control and a negative reference in an assay plate. For hit selection, the size of effects of a compound (i.e., a small molecule or an siRNA) is represented by the magnitude of difference between the compound and a negative reference ...
In statistics, an effect size is a value measuring the strength of the relationship between two variables in a population, or a sample-based estimate of that quantity. It can refer to the value of a statistic calculated from a sample of data, the value of one parameter for a hypothetical population, or to the equation that operationalizes how statistics or parameters lead to the effect size ...
The finite difference of higher orders can be defined in recursive manner as Δ n h ≡ Δ h (Δ n − 1 h) . Another equivalent definition is Δ n h ≡ [T h − I ] n . The difference operator Δ h is a linear operator, as such it satisfies Δ h [ α f + β g ](x) = α Δ h [ f ](x) + β Δ h [g](x) . It also satisfies a special Leibniz rule:
The word liposome derives from two Greek words: lipo ("fat") and soma ("body"); it is so named because its composition is primarily of phospholipid.. Liposomes were first described by British hematologist Alec Douglas Bangham [10] [11] [12] in 1961 at the Babraham Institute, in Cambridge—findings that were published 1964.