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Glacial acetic acid is used in analytical chemistry for the estimation of weakly alkaline substances such as organic amides. Glacial acetic acid is a much weaker base than water, so the amide behaves as a strong base in this medium. It then can be titrated using a solution in glacial acetic acid of a very strong acid, such as perchloric acid. [52]
Phase behavior Triple point: 289.8 K (16.7 °C), ? Pa Critical point: 593 K (320 °C), 57.8 bar Eutectic point with water –26.7 °C Std enthalpy change
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However, acetic acid is usually also formed as a by-product of the reaction, so that the solvent is ultimately a mixture of methylene chloride, acetic anhydride and acetic acid. A very rare heterogeneous process is the fiber acetate process, which is only used for the production of cellulose triacetate as an end product.
If the glacial acetic acid is replaced by 5 ml of formalin (37–40% formaldehyde), the resulting solution is Helly's fixative, also sometimes called "formol-Zenker".Helly is stable for only a few hours because the formaldehyde and dichromate components react, producing formic acid and chromium(III) ions; the orange solution becomes greenish.
TAE buffer is commonly prepared as a 50× stock solution for laboratory use. A 50× stock solution can be prepared by dissolving 242 g Tris base in water, adding 57.1 ml glacial acetic acid, and 100 ml of 500 mM EDTA (pH 8.0) solution, and bringing the final volume up to 1 litre.
Sodium acetate (anhydrous) is widely used as a shelf-life extending agent and pH-control agent. [10] ... A solution of sodium acetate (a basic salt of acetic acid) ...
An example of triglyceride occurring in the saponifiable fraction of oils with a saturated fatty acid residue, a monounsaturated fatty acid residue and a triple unsaturated (polyunsaturated) fatty acid residue. The triple esterified glycerol (marked black) can be seen in the center of the structure. Such a triglyceride has a high iodine value ...