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Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light , and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
Axial bright-field detectors are located in the centre of the cone of illumination of the transmitted beam, and are often used to provide complementary images to those obtained by ADF imaging. [12] Annular bright-field detectors, located within the cone of illumination of the transmitted beam, have been used to obtain atomic resolution images ...
Microscope-based diagnostics are widely performed and served as a gold standard in histological analysis. However this procedure generally requires a series time-consuming lab-based procedures including fixation, paraffin embedment, sectioning, and staining to produce microscope slides with optically thin tissue slides (4–6 μm).
Confocal microscope images of a tomato leaf from Solanum lycopersicum. Brightfield DIC image showing guard cells and pavement cells (above). Same region showing Chlorophyll A autofluorescence with 440 nm laser excitation and far red emission (below). Microscopic images of a moss leaf from Plagiomnium undulatum.
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
Download as PDF; Printable version; In other projects Wikidata item ... Brightfield may refer to: Bright-field microscopy; Solar array built on a solar landfill or ...
Download as PDF; Printable version; In other projects ... Appearance. move to sidebar hide. Bright field may refer to: MV Bright Field, a bulk ... Bright-field microscopy
An annular dark-field image taken may be complementary to a bright-field image constructed from the captured CBED images. The use of a hollow detector with a hole in the middle can allow for transmitted electrons to be passed to an EELS detector while scanning.