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In a 1945 study by Demerec and Fano, [4] T7 was used to describe one of the seven phage types (T1 to T7) that grow lytically on Escherichia coli. [5] Although all seven phages were numbered arbitrarily, phages with odd numbers, or T-odd phages, were later discovered to share morphological and biochemical features that distinguish them from T-even phages. [6]
Italiano: Rappresentazione schematica della struttura del fago T7. Español: Representación esquemática de la estructura del fago T7. Français : Représentation schématique de la structure de la phage T7.
T7 phage group was renamed to T7-like phages in the sixth report in 1995. In 1998, the whole family was moved into the newly created order Caudovirales , and the genus was renamed again in the seventh report in 1999 to T7-like viruses . 2009 saw the genus moved into the newly created subfamily Autographivirinae , and it was renamed again in ...
In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different polymerases.
Once this has occurred, the prohead undergoes maturation by cleavage of capsid subunits to form an icosahedral phage head with 5-fold symmetry. After the head maturation, the tail is joined in one of two ways: Either the tail is constructed separately, and joined with the connector, or the tail is constructed directly onto the phage head.
Podoviridae was a family of bacteriophage in the order Caudovirales often associated with T-7 like phages. [1] The family and order Caudovirales have now been abolished, with the term podovirus now used to refer to the morphology of viruses in this former family. [2]
However, according to the U.S. Department of Agriculture's FoodData Central database, as of a few years ago, more than 8,000 branded food products still contained Red Dye No. 3. Common food ...
The two ends of the phage are capped by a few copies of proteins that are important for infection of the host bacteria, and also for assembly of nascent phage particles. These proteins are the products of phage genes 3 and 6 at one end of the phage, and phage genes 7 and 9 at the other end.