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Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
The plunger is again depressed to the first stop, and then to the second stop, or 'blowout', position. This action will fully evacuate the tip and dispense the liquid. In an adjustable pipette, the volume of liquid contained in the tip is variable; it can be changed via a dial or other mechanism, depending on the model.
For example, a motorized pipette controller can aid liquid aspiration or dispensing using volumetric pipettes or graduated pipettes; [13] a tablet can interact in real-time with the pipette and guide a user through a protocol; [14] and a pipette station can help to control the pipette tip immersion depth and improve ergonomics.
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A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
The Zero Gravity Research Facility was built in 1966 as part of NASA's Centaur upper-stage rocket development program. In order to ensure proper firing and functioning of upper-stage rockets, NASA needed to understand the behavior of fluids (importantly, the liquid gases fueling the rockets), in the reduced gravity where they would fire.
Steps for operating a positive displacement pipette [15] Set the pipetting volume. Attach a CP tip onto the pipette. Hold the pipette vertically and press the plunger to the first stop. Put the CP tip into the sample and slowly release it, moving the button to the home position. Press the plunger to the first stop again to dispense the sample.