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  2. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.

  3. Time-lapse microscopy - Wikipedia

    en.wikipedia.org/wiki/Time-lapse_microscopy

    This is known as live-cell imaging. A few tools have been developed to identify and analyze single cells during live-cell imaging. [2] [3] [4] Time-lapse microscopy is the method that extends live-cell imaging from a single observation in time to the observation of cellular dynamics over long periods of time.

  4. Scanning ion-conductance microscopy - Wikipedia

    en.wikipedia.org/wiki/Scanning_ion-conductance...

    Scanning ion-conductance microscopy diagram. Scanning ion-conductance microscopy (SICM) is a scanning probe microscopy technique that uses an electrode as the probe tip. [1] SICM allows for the determination of the surface topography of micrometer and even nanometer-range [2] structures in aqueous media conducting electrolytes. The samples can ...

  5. Live single-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live_single-cell_imaging

    In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...

  6. Lattice light-sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Lattice_light-sheet_microscopy

    Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...

  7. Multifocal plane microscopy - Wikipedia

    en.wikipedia.org/wiki/Multifocal_plane_microscopy

    The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM), also known as multiplane microscopy or multifocus microscopy, is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.

  8. Coherent Raman scattering microscopy - Wikipedia

    en.wikipedia.org/wiki/Coherent_Raman_scattering...

    Coherent Raman scattering (CRS) microscopy is a multi-photon microscopy technique based on Raman-active vibrational modes of molecules. The two major techniques in CRS microscopy are stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS). SRS and CARS were theoretically predicted and experimentally realized in the 1960s.

  9. 4D scanning transmission electron microscopy - Wikipedia

    en.wikipedia.org/wiki/4D_scanning_transmission...

    4D scanning transmission electron microscopy (4D STEM) is a subset of scanning transmission electron microscopy (STEM) which utilizes a pixelated electron detector to capture a convergent beam electron diffraction (CBED) pattern at each scan location. This technique captures a 2 dimensional reciprocal space image associated with each scan point ...