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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry , with applications in research and clinical practice.
Through the work of Karl von Vierordt, Louis-Charles Malassez, Karl Bürker and others blood cell concentration could by the late 19th century be accurately measured using a blood cell counting chamber, the hemocytometer, and an optical microscope. [3] [4] Until the 1950s the hemocytometer was the standard method to count blood cells. [5]
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]
A complete blood count (CBC), also known as a full blood count (FBC), is a set of medical laboratory tests that provide information about the cells in a person's blood.The CBC indicates the counts of white blood cells, red blood cells and platelets, the concentration of hemoglobin, and the hematocrit (the volume percentage of red blood cells).
Coulter principle — the transient current drop is proportional to the particle volume The tip of the Coulter counter in a buffer solution, counting cells in solution.. A Coulter counter [1] [2] is an apparatus for counting and sizing particles suspended in electrolytes.
MCHC can be normal even when hemoglobin production is decreased (such as in iron deficiency) due to a calculation artifact. MCHC can be elevated ("hyperchromic") in hereditary spherocytosis, sickle cell disease and homozygous hemoglobin C disease, depending upon the hemocytometer. [4] [5] MCHC can be elevated in some megaloblastic anemias. MCHC ...
MDCalc is a free online medical reference for healthcare professionals that provides point-of-care clinical decision-support tools, including medical calculators, scoring systems, and algorithms. [1]