Search results
Results From The WOW.Com Content Network
Promoters are located near the transcription start sites of genes, upstream on the DNA (towards the 5' region of the sense strand). Promoters can be about 100–1000 base pairs long, the sequence of which is highly dependent on the gene and product of transcription, type or class of RNA polymerase recruited to the site, and species of organism ...
Promoter activity of the P-RM and P-R promoters vs RNA polymerase concentration in the enterobacteriophage lambda [1]. Promoter activity is a term that encompasses several meanings around the process of gene expression from regulatory sequences —promoters [2] and enhancers. [3]
The promoter region is a prime regulator of transcription. Promoter regions regulate transcription of all genes within bacteria. As a result of their involvement, the sequence of base pairs within the promoter region is significant; the more similar the promoter region is to the consensus sequence, the tighter RNA polymerase will be able to bind.
RNA polymerase binding in bacteria involves the sigma factor recognizing the core promoter region containing the −35 and −10 elements (located before the beginning of sequence to be transcribed) and also, at some promoters, the α subunit C-terminal domain recognizing promoter upstream elements. [12]
Cis-regulatory DNA sequences that are located in DNA regions distant from the promoters of genes can have very large effects on gene expression, with some genes undergoing up to 100-fold increased expression due to such a cis-regulatory sequence. [36] These cis-regulatory sequences include enhancers, silencers, insulators and tethering elements ...
The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. [ 1 ] [ 2 ] In E. coli , the P BAD promoter is adjacent to the P C promoter (systematically araCp ), which transcribes the araC gene in the opposite direction.
Get AOL Mail for FREE! Manage your email like never before with travel, photo & document views. Personalize your inbox with themes & tabs. You've Got Mail!
The tac promoter consists of the '–35' region of the trp promoter and the '–10' region of the lac promoter (and differs from a related trc promoter by 1 bp [3]). The tac promoter is, therefore, inducible by IPTG (Isopropyl β- D -1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp promoters.