Search results
Results From The WOW.Com Content Network
Micellar electrokinetic chromatography (MEKC) is a chromatography technique used in analytical chemistry. It is a modification of capillary electrophoresis (CE), extending its functionality to neutral analytes, [ 1 ] where the samples are separated by differential partitioning between micelles (pseudo-stationary phase) and a surrounding aqueous ...
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
Some capillary techniques provide also a chamber like arrangement, however, there is no filter between the cells and the test substance. [5] Quantitative results are gained by the multiwell type of this probe using 4-8-12-channel pipettes. Accuracy of the pipette and increased number of the parallel running samples is the great advantage of ...
Electro-osmotic flow is most significant when in small channels, and is an essential component in chemical separation techniques, notably capillary electrophoresis. Electro-osmotic flow can occur in natural unfiltered water, as well as buffered solutions. Electro-osmotic flow schematic
Buffer transfer by capillary action from a region of high water potential to a region of low water potential (usually filter paper and paper tissues) is then used to move the DNA from the gel onto the membrane; ion exchange interactions bind the DNA to the membrane due to the negative charge of the DNA and positive charge of the membrane.
A type of ion exchange chromatography, membrane exchange [39] [40] is a relatively new method of purification designed to overcome limitations of using columns packed with beads. Membrane Chromatographic [ 41 ] [ 42 ] devices are cheap to mass-produce and disposable unlike other chromatography devices that require maintenance and time to ...
The stopped-flow method evolved from the continuous-flow technique developed by Hamilton Hartridge and Francis Roughton [7] to study the binding of oxygen to hemoglobin. In the continuous-flow system, the reaction mixture was passed through a long tube, past an observation system (a simple colorimeter in 1923), and then discarded as waste.
Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. However, the entire process is commonly referred to as northern blotting. [5] The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford ...