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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg 2+.
The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...
Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School ...
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.
New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. [3]
The instructions state that medical experimentation would be prohibited if: the subject is a minor. the subject has not provided unambiguous consent. possible negative consequences have not been explained. there is no authorization from the medical director. These instructions were not committed to law and as such were not legally binding.
A rape kit or rape test kit is a package of items used by medical, police or other personnel for gathering and preserving physical evidence following an instance or allegation of sexual assault. The evidence collected from the victim can aid the criminal rape investigation and the prosecution of a suspected assailant.