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Enrichment culture is the use of certain growth media to favor the growth of a particular microorganism over others, enriching a sample for the microorganism of interest. . This is generally done by introducing nutrients or environmental conditions that only allow the growth of an organism of inte
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Chocolate agar showing Francisella tularensis colonies Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, the nonselective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of N. gonorrhoeae, while the selective Thayer–Martin medium on the right, containing ...
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
More generally, enriched media is an agar variety that is infused with the necessary nutrients required by fastidious organisms to grow. Despite the large diversity of agar mediums, yeast extract is a common ingredient across all varieties as it is a macronutrient that provides a nitrogen source for all bacterial cell types.
Streak plates of several bacterial species on nutrient agar plates. Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms.
Thioglycolate broth is a multipurpose, enrichment, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes. [1]
An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3).. Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology.