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Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. [1] This takes place through a pilus .
The nucleophilic lysine residue is commonly targeted site in protein bioconjugation, typically through amine-reactive N-hydroxysuccinimidyl (NHS) esters. [3] To obtain optimal number of deprotonated lysine residues, the pH of the aqueous solution must be below the pKa of the lysine ammonium group, which is around 10.5, so the typical pH of the reaction is about 8 and 9.
Triparental mating is a form of bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. [1] Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis ...
Although ICEs exhibit various mechanisms promoting their integration, transfer and regulation, they share many common characteristics. ICEs comprise all mobile genetic elements with self-replication, integration, and conjugation abilities, including conjugative transposons, regardless of the particular conjugation and integration mechanism by which they act.
Conjugation is followed by one or more "exconjugant divisions." [34] Stages of conjugation Stages of conjugation in Paramecium caudatum. In Paramecium caudatum, the stages of conjugation are as follows (see diagram at right): Compatible mating strains meet and partly fuse; The micronuclei undergo meiosis, producing four haploid micronuclei per ...
Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host ...
Different forms of isogamy: A) isogamy of motile cells, B) isogamy of non-motile cells, C) conjugation (isogamy in the broad sense). Isogamy is a form of sexual reproduction that involves gametes of the same morphology (indistinguishable in shape and size), and is found in most unicellular eukaryotes. [1]
The final result of conjugation, transduction, and/or transformation is the production of genetic recombinants, individuals that carry not only the genes they inherited from their parent cells but also the genes introduced to their genomes by conjugation, transduction, and/or transformation. [5] [6] [7]