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Bioconjugation is a chemical strategy to form a stable covalent link between two molecules, at least one of which is a biomolecule. Methods to conjugate biomolecules ...
bioconjugation, for example, azidocoumarin, and; biomaterials [62] In combination with combinatorial chemistry, high-throughput screening, and building chemical libraries, click chemistry has hastened new drug discoveries by making each reaction in a multistep synthesis fast, efficient, and predictable.
One conjugate, consisting out of an EGFR Nanobody and a siRNA being combined through maleimide bioconjugation, proves the possibility of successful delivery of ONs by nanobodies. [ 21 ] Another example consists out of an anti- CD71 Fab fragment which was conjugated to a maleimide bearing siRNA (itself having 2’OMe/2’F modifications and ...
Bioconjugate Chemistry is a monthly peer-reviewed scientific journal covering research in bioconjugation and the interface between man-made and biological materials. The journal was established in 1990 and is published by the American Chemical Society.
The Staudinger ligation is a reaction developed by the Bertozzi group in 2000 that is based on the classic Staudinger reaction of azides with triarylphosphines. [15] It launched the field of bioorthogonal chemistry as the first reaction with completely abiotic functional groups although it is no longer as widely used.
Of interest in chemical biology is the Staudinger ligation, which has been called one of the most important bioconjugation methods. [5] Two versions of the Staudinger ligation have been developed. Both begin with the classic iminophosphorane reaction. In classical Staudinger ligation, the organophosphorus compound becomes incorporated into the ...
SMCC is often used in bioconjugation to link proteins with other functional entities (fluorescent dyes, tracers, nanoparticles, cytotoxic agents). [1] For example, a targeted anticancer agent – trastuzumab emtansine ( antibody-drug conjugate containing an antibody trastuzumab chemically linked to a highly potent drug DM-1 ) – is prepared ...
An improved synthetic approach to a monofluorosubstituted cyclooctyne (MFCO) was introduced that could easily be converted to a useful reactive intermediate for bioconjugation applications, although the reactivity was somewhat slower than DIFO. The MFCO demonstrated excellent stability characteristics for long-term storage. [2]