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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
The vast majority of liquid chromatographic systems are equipped with ultraviolet (UV) absorption detectors. The most common UV-Vis detectors used are variable wavelength detectors (VWD), photo diode array detectors (PDA), and diode array detectors (DAD). [4] Variable wavelength detectors decide in advance which wavelength is needed for the ...
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
Approximately 30,000 DU spectrophotometers were manufactured and sold between 1941 and 1976. Sometimes referred to as a UV–Vis spectrophotometer because it measured both the ultraviolet (UV) and visible spectra, the DU spectrophotometer is credited as being a
UV-Vis absorption SEC is a recent technique that is continuously evolving. However, many advantages have been observed over other techniques. However, many advantages have been observed over other techniques.
Developed by Bausch & Lomb and launched in 1953, the Spectronic 20 was the first low-cost spectrophotometer. [5] It rapidly became an industry standard [7] due to its low cost, durability and ease of use, [8] and has been referred to as an "iconic lab spectrophotometer". [9] Approximately 600,000 units were sold over its nearly 60 year ...