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The first real time (video rate) Ca 2+ imaging was carried out in 1986 in cardiac cells using intensified video cameras. [3] Later development of the technique using laser scanning confocal microscopes revealed sub-cellular Ca 2+ signals in the form of Ca 2+ sparks and Ca 2+ blips.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Zoom lenses (sometimes referred to as "true" zoom) are ideally parfocal, in that focus is maintained as the lens is zoomed (i.e., focal length and magnification changed), which is convenient and has the advantage of allowing more accurate focusing at maximal focal length then zooming back to a shorter focal length to compose the image.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
Prior to recent advances in virtual microscopy, slides were commonly digitized by various forms of film scanner and image resolutions rarely exceeded 5000 dpi. Nowadays, it is possible to achieve more than 100,000 dpi and thus resolutions approaching that visible under the optical microscope. This increase in scanning resolution comes at a ...
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