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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment.It is an important subset of cytometry, with applications in research and clinical practice.
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]
The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent), and stains the nuclei of the white blood cells, making them easier to see and count. [1] Türk's solution is intended for use in determining total leukocyte count in a defined volume of blood.
Cytometers are the instruments which count the blood cells in the common blood test.. Cytometry is the measurement of number and characteristics of cells.Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the ...
Previously, this procedure involved preparing a peripheral blood smear and manually counting each type of cell under a microscope, a process that typically required a half-hour. A Coulter counter played an important role in the development of the first cell sorter , and was involved in the early development of flow cytometry .
Digital holographic microscopy makes it possible to perform cell counting and to measure cell viability directly in the cell culture chamber. [15] [16] Today, the most commonly used cell counting methods, hemocytometer or Coulter counter, only work with cells that are in suspension. Label-free viability analysis of adherent cell cultures.
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