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Acid–base extraction is a subclass of liquid–liquid extractions and involves the separation of chemical species from other acidic or basic compounds. [1] It is typically performed during the work-up step following a chemical synthesis to purify crude compounds [2] and results in the product being largely free of acidic or basic impurities.
Laboratory-scale liquid-liquid extraction. Photograph of a separatory funnel in a laboratory scale extraction of 2 immiscible liquids: liquids are a diethyl ether upper phase, and a lower aqueous phase. Soxhlet extractor. Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. The distribution ...
changing the protonation state of the products or impurities by adding an acid or base. separating the reaction mixture into organic and aqueous layers by liquid-liquid extraction. removal of solvents by evaporation. purification by chromatography, distillation or recrystallization.
A separatory funnel used for liquid–liquid extraction, as evident by the two immiscible liquids.. Liquid–liquid extraction, also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar).
Liquid–liquid extraction removes an impurity or recovers a desired product by dissolving the crude material in a solvent in which other components of the feed material are soluble. Crystallization separates a product from a liquid feed stream, often in extremely pure form, by cooling the feed stream or adding precipitants that lower the ...
Acid–base extraction; Affinity electrophoresis; Affinity magnetic separation; Air-free technique; Ames test; Amplicon; Analytical band centrifugation; Analytical ultracentrifugation; Animal testing; Annexin A5 affinity assay; Anterograde tracing; Allele-specific oligonucleotide; Artificial cerebrospinal fluid; Assay
A good example of an incomplete separation technique is oil refining. Crude oil occurs naturally as a mixture of various hydrocarbons and impurities. The refining process splits this mixture into other, more valuable mixtures such as natural gas , gasoline and chemical feedstocks , none of which are pure substances, but each of which must be ...
Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology. It is widely used for isolating RNA (as well as DNA and protein in some cases).