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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
Species identification hierarchy in clinical settings. Historically, the kingdom Monera was divided into four divisions based primarily on Gram staining: Bacillota (positive in staining), Gracilicutes (negative in staining), Mollicutes (neutral in staining) and Mendocutes (variable in staining). [4]
Staining used in microbiology identification include: Gram stain, Acid-fast stain, Giemsa stain, ... This is also the key method for Sanger sequencing. Fluorescent ...
In Berlin, in 1884, Gram developed a method for distinguishing between two major classes of bacteria. [1] This technique, known as Gram staining, continues to be a standard procedure of medical microbiology. This work gained Gram an international reputation. The staining method later played a major role in classifying bacteria. Gram was a ...
Depending on the nature of infection, an appropriate specimen is collected and sent to a bacteriology laboratory for identification. As with most bacteriological specimens, a Gram stain is performed, which may show Gram-negative rods and/or white blood cells.
Staphylococcus saprophyticus; S. saprophyticus on Mueller–Hinton agar exhibiting resistance to novobiocin characteristic for species identification : Gram stain of a Gram positive S. saprophyticus sample suspension, lab cultured on Tryptic Soy agar (TSA)
Laboratory smears show Gram-positive rods (though Gram stain has low sensitivity for this microbe). It is nonmotile, catalase-negative, microaerophilic, capnophilic, and non-spore-forming. It can also produce H 2 S (gas), which is a unique characteristic for a Gram-positive bacillus. Acid is produced from glucose, fructose, galactose, and ...
Streptococcus agalactiae (also known as group B streptococcus or GBS) is a gram-positive coccus (round bacterium) with a tendency to form chains (as reflected by the genus name Streptococcus). It is a beta- hemolytic , catalase -negative, and facultative anaerobe .