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The S2 cells have been shown to grow up to 5.1×10 7 cells/ml in serum free medium [3] and above 10 7 cells/ml in basal media such as that used in Schneider's experiments. [4] ExpreS2ion Biotechnologies have shown a cell concentration of up 7.0x10 7 cell/ml. [ 5 ]
The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...
Since the original CHO cell line was described in 1956, many variants of the cell line have been developed for various purposes. [10] [additional citation(s) needed] In 1957, CHO-K1 was generated from a single clone of CHO cells. [16] According to an industry source, however, scientist Theodore Puck first isolated CHO-K1 in 1968. [1]
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This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the ...
U-937 cells are a pro-monocytic model cell line used in biomedical research. [1] They were isolated from the histiocytic lymphoma of a 37-year-old male patient in 1974. [2] Due to the relative uniformity of expanded cultures and lower maintenance requirements these cells have been since used as an important tool for studying phagocyte differentiation and different kinds of cell-to-cell ...