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[1]) is a water-soluble sodium salt of Alizarin sulfonic acid with a chemical formula of C 14 H 7 NaO 7 S. [2] [1] Alizarin Red S was discovered by Graebe and Liebermann in 1871. [2] In the field of histology alizarin Red S is used to stain calcium deposits in tissues, [3] [4] and in geology to stain and differentiate carbonate minerals. [3]
A diaphonized mirror dory.The bones are dyed red and the cartilage is dyed blue. Diaphonized veiled chameleon. Field Museum of Natural History, Chicago.. Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the animal transparent by bathing it in trypsin, and then stains the bones and cartilage ...
Free (ionic) calcium forms precipitates with alizarin, and tissue block containing calcium stain red immediately when immersed in alizarin. Thus, both pure calcium and calcium in bones and other tissues can be stained. These alizarin-stained elements can be better visualized under fluorescent lights, excited by 440–460 nm. [18]
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