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Dry heat sterilization of an object is one of the earliest forms of sterilization practiced. It uses hot air that is either free from water vapor or has very little of it, where this moisture plays a minimal or no role in the process of sterilization.
The standard settings for a hot air oven are: 1.5 to 2 hours at 160 °C (320 °F) 6 to 12 minutes at 190 °C (374 °F)....plus the time required to preheat the chamber before beginning the sterilization cycle. If the door is opened before time, heat escapes and the process becomes incomplete.
Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [ 1 ] [ 2 ] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores , which can germinate shortly thereafter and resume growth.
Freshly baked bread Anders Zorn – Bread baking (1889) Baking is a method of preparing food that uses dry heat, typically in an oven, but can also be done in hot ashes, or on hot stones. The most common baked item is bread, but many other types of foods can be baked. [1]
This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Heat is applied by baking in a dry heat oven that is designed specifically for the depyrogenation process. Although endotoxins are relatively thermally stable, sufficient heating (250 °C for 30 min) results in a 3-log reduction of endotoxin levels.
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See also References Further reading External links A acidulate To use an acid (such as that found in citrus juice, vinegar, or wine) to prevent browning, alter flavour, or make an item safe for canning. al dente To cook food (typically pasta) to the point where it is tender but not mushy. amandine A culinary term indicating a garnish of almonds. A dish served amandine is usually cooked with ...