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In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor R F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [2]
The retention factor (R ƒ) may be defined as the ratio of the distance travelled by the solute to the distance travelled by the solvent.It is used in chromatography to quantify the amount of retardation of a sample in a stationary phase relative to a mobile phase. [2]
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
The [N 1/2 /4] term is the column factor, the [(α-1)/α] term is the thermodynamic factor, and the [k 2 '/(1+k 2 ')] term is the retention factor. The 3 factors are not completely independent, but they are very close, and can be treated as such.
The retardation factor (R f), or retention factor, quantifies the results. It is the distance traveled by a given substance divided by the distance traveled by the mobile phase. [citation needed] Development of a TLC plate. Spots that appear purple separate into red spots and blue spots.
Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]
Retention distance, or R D, is a concept in thin layer chromatography, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response functions. It is calculated from the following formula:
The Purnell equation is an equation used in analytical chemistry to calculate the resolution R s between two peaks in a chromatogram. [1] [2]= (′ + ′) where R s is the resolution between the two peaks