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With any telescope, microscope or lens, a maximum magnification exists beyond which the image looks bigger but shows no more detail. It occurs when the finest detail the instrument can resolve is magnified to match the finest detail the eye can see. Magnification beyond this maximum is sometimes called "empty magnification".
The total angular magnification of a microscope image is then simply calculated by multiplying the eyepiece power by the objective power. For example, a 10× eyepiece with a 40× objective will magnify the image 400 times.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
The eye relief of an optical instrument (such as a telescope, a microscope, or binoculars) is the distance from the last surface of an eyepiece within which the user's eye can obtain the full viewing angle. If a viewer's eye is outside this distance, a reduced field of view will be obtained.
The effective focal length is the inverse of the optical power of an optical system, and is the value used to calculate the magnification of the system. [1] The imaging properties of the optical system can be modeled by replacing the system with an ideal thin lens with the same EFL. [2]
The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly ...
By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...
In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by