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  2. Protoplast - Wikipedia

    en.wikipedia.org/wiki/Protoplast

    Protoplast (from Ancient Greek πρωτόπλαστος (prōtóplastos) 'first-formed'), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. [ 1 ] [ 2 ] Protoplasts can be generated by stripping the cell wall from plant , [ 3 ] bacterial , [ 4 ] [ 5 ] or fungal cells [ 5 ] [ 6 ] by mechanical ...

  3. Micropropagation - Wikipedia

    en.wikipedia.org/wiki/Micropropagation

    Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.

  4. Somatic fusion - Wikipedia

    en.wikipedia.org/wiki/Somatic_fusion

    Fused protoplast (left) with chloroplasts (from a leaf cell) and coloured vacuole (from a petal) Somatic fusion, also called protoplast fusion, is a type of genetic modification in plants by which two distinct species of plants are fused together to form a new hybrid plant with the characteristics of both, a somatic hybrid. [1]

  5. Electroporation - Wikipedia

    en.wikipedia.org/wiki/Electroporation

    Electroporation is also highly efficient for the introduction of foreign genes into tissue culture cells, especially mammalian cells. For example, it is used in the process of producing knockout mice, as well as in tumor treatment, gene therapy, and cell-based therapy.

  6. Somatic embryogenesis - Wikipedia

    en.wikipedia.org/wiki/Somatic_embryogenesis

    Many culture systems induce and maintain somatic embryogenesis by continuous exposure to 2,4-dichlorophenoxyacetic acid. Abscisic acid has been reported to induce somatic embryogenesis in seedlings. After callus formation, culturing on a low auxin or hormone free media will promote somatic embryo growth and root formation.

  7. Plant tissue culture - Wikipedia

    en.wikipedia.org/wiki/Plant_tissue_culture

    Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .

  8. L-form bacteria - Wikipedia

    en.wikipedia.org/wiki/L-form_bacteria

    L-form strains can be unstable, tending to revert to the normal form of the bacteria by regrowing a cell wall, but this can be prevented by long-term culture of the cells under the same conditions that were used to produce them – letting the wall-disabling mutations to accumulate by genetic drift. [9]

  9. Protoplast (religion) - Wikipedia

    en.wikipedia.org/wiki/Protoplast_(religion)

    A protoplast, from ancient Greek πρωτόπλαστος (prōtóplastos, "first-formed"), in a religious context initially referred to the first human [1] or, more generally, to the first organized body of progenitors of humankind (as in Adam and Eve or Manu and Shatrupa), or of surviving humanity after a cataclysm (as in Deucalion or Noah).