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The cellular processes of DNA replication and transcription involve DNA and RNA synthesis, respectively. DNA synthesis uses dNTPs as substrates, while RNA synthesis uses rNTPs as substrates. [2] NTPs cannot be converted directly to dNTPs. DNA contains four different nitrogenous bases: adenine, guanine, cytosine and thymine.
A ribonucleotide tri-phosphate (rNTP) is composed of a ribose sugar, 3 phosphate groups attached via diester bonds to the 5' oxygen on the ribose and a nitrogenous base attached to the 1' carbon on the ribose. rNTP's are also referred to as NTPs while the deoxyribose version is referred to as dNTPs.
This is an accepted version of this page This is the latest accepted revision, reviewed on 16 January 2025. Cell division producing haploid gametes For the figure of speech, see Meiosis (figure of speech). For the process whereby cell nuclei divide to produce two copies of themselves, see Mitosis. For excessive constriction of the pupils, see Miosis. For the parasitic infestation, see Myiasis ...
Pre-labelling → involves the use of 32 P: cells are grown in 32 P containing medium, thus allowing the incorporation of [α-32 P]NTPs during transcription by T7 RNA polymerase. The modified RNA is then extracted, and each RNA species is isolated and subsequently digested by T2 RNase.
Meiosis involves two rounds of nuclear division, not just one. Prior to undergoing meiosis, a cell goes through an interphase period in which it grows, replicates its chromosomes, and checks all of its systems to ensure that it is ready to divide. Like mitosis, meiosis also has distinct stages called prophase, metaphase, anaphase, and telophase ...
Interphase is the process through which a cell must go before mitosis, meiosis, and cytokinesis. [15] Interphase consists of three main phases: G 1 , S , and G 2 . G 1 is a time of growth for the cell where specialized cellular functions occur in order to prepare the cell for DNA replication. [ 16 ]
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Eukaryotic Transcription. Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of transportable complementary RNA replica. [1] Gene transcription occurs in both eukaryotic and prokaryotic cells. Unlike prokaryotic RNA polymerase that initiates the transcription of all ...