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Fluorescent lighting is more energy-efficient than incandescent lighting elements. However, the uneven spectrum of traditional fluorescent lamps may cause certain colors to appear different from when illuminated by incandescent light or daylight. The mercury vapor emission spectrum is dominated by a short-wave UV line at 254 nm (which provides ...
Multicolor fluorescence image of living HeLa cells. Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy.
A color chart or color reference card is a flat, physical object that has many different color samples present. They can be available as a single-page chart, or in the form of swatchbooks or color-matching fans. Typically there are two different types of color charts: Color reference charts are intended
A multispectral image of tissue from a mouse intestine, showing how autofluoresce can obscure several fluorescence signals. Autofluorescence can be problematic in fluorescence microscopy. Light-emitting stains (such as fluorescently labelled antibodies) are applied to samples to enable visualisation of specific structures.
Similarly, color is due to the energy absorbed by the compound, when an electron transitions from the HOMO to the LUMO. Lycopene is a classic example of a compound with extensive conjugation (11 conjugated double bonds), giving rise to an intense red color (lycopene is responsible for the color of tomatoes).
Atomic Fluorescence Spectroscopy (AFS) techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media, such as CVAFS which is used for heavy metals detection, such as mercury. Fluorescence can also be used to redirect photons, see fluorescent solar collector.
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Spectrofluorometers, developed in the mid-1980s, made it possible to perform advanced analysis of fluorescence. Filter fluorometers and spectrofluorometers identify the intensity of fluorescence that is present in a liquid sample. Different dyes and chemicals produce a distinctive wavelength that is determined during analysis.