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The most useful stain in the diagnosis of amyloid is Congo red, which, combined with polarized light, makes the amyloid proteins appear apple-green on microscopy. Also, thioflavin T stain may be used. [22] A number of imaging techniques such as a Nuclear Medicine PYP scan, DPD scan or SAP scan are also in use. [23]
In the clinical setting, amyloid diseases are typically identified by a change in the spectroscopic properties of planar aromatic dyes such as thioflavin T, congo red or NIAD-4. [74] In general, this is attributed to the environmental change, as these dyes intercalate between β-strands to confine their structure. [75]
Due to a color change from blue to red at pH 3.0–5.2, Congo red can be used as a pH indicator. Since this color change is an approximate inverse of that of litmus, it can be used with litmus paper in a simple parlor trick: add a drop or two of Congo red to both an acid solution and a base solution. Dipping red litmus paper in the red solution ...
Paget's disease (breast and extramammary) Cytokeratin 20: Extramammary Paget's disease of endodermal origin Merkel cell carcinoma: Colloidal iron: Papular mucinosis Scleredema Pretibial myxedema: Congo red: Amyloidosis Colloid milium: Crystal violet: Amyloidosis Colloid milium: D2-40: Lymphangioma circumscriptum: de Galantha: Pseudogout: Dylon ...
One such stain is Congo Red, which binds specifically to the amyloid deposit and can be characterized by various lighting methods. [7] Under polarized light, the amyloid deposits while show pathognomonic apple green birefringence, and under plain light the deposits will appear a light salmon pink color. [7] Familial amyloidosis symptoms are ...
Cerebral amyloid angiopathy (CAA) is a form of angiopathy in which amyloid beta peptide deposits in the walls of small to medium blood vessels of the central nervous system and meninges. [ 2 ] [ 3 ] The term congophilic is sometimes used because the presence of the abnormal aggregations of amyloid can be demonstrated by microscopic examination ...
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