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Definition of Spawn: Spawn is a type of medium present in mushroom tissue that propagates the fungus such as Trichoderma which is the root system of mushrooms. [ 5 ] Mycelium, or actively growing mushroom culture, is placed on a substrate—usually sterilized grains such as rye or millet—and induced to grow into those grains.
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The inoculation needle after incineration is cooled down on an uninoculated region of the agar plate culture. [1] Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. [1] The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced.
Inoculation is the act of implanting a pathogen or other microbe or virus into a person or other organism. It is a method of artificially inducing immunity against ...
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Serial passage is the process of growing bacteria or a virus in iterations. For instance, a virus may be grown in one environment, and then a portion of that virus population can be removed and put into a new environment.
Life cycle of corn grey leaf spot. Cercospora zeae-maydis survives only as long as infected corn debris is present; however, it is a poor soil competitor. The debris on the soil surface is a cause for primary inoculation that infects the incoming corn crop for the next season.
Fungal inoculation alone can benefit host plants. Inoculation paired with other amendments can further improve conditions. Arbuscular mycorrhizal inoculation combined with compost is a common household amendment for personal gardens, agriculture, and nurseries.