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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
This is also known as inoculation, spawning or adding spawn. Its main advantages are to reduce chances of contamination while giving mushrooms a firm beginning. [3] [4] Definition of Spawn: Spawn is a type of medium present in mushroom tissue that propagates the fungus such as Trichoderma which is the root system of mushrooms. [5]
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Another way of plating organisms, next to streaking, on agar plates is the spot analysis. This type of analysis is often used to check the viability of cells and is performed with pinners (often also called froggers). A third technique is using sterile glass beads to plate out cells.
The inoculation needle after incineration is cooled down on an uninoculated region of the agar plate culture. [1] Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. [1] The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced.
Fungal inoculation alone can benefit host plants. Inoculation paired with other amendments can further improve conditions. Arbuscular mycorrhizal inoculation combined with compost is a common household amendment for personal gardens, agriculture, and nurseries.
Life cycle of corn grey leaf spot. Cercospora zeae-maydis survives only as long as infected corn debris is present; however, it is a poor soil competitor. The debris on the soil surface is a cause for primary inoculation that infects the incoming corn crop for the next season.
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle