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  2. Protoplast - Wikipedia

    en.wikipedia.org/wiki/Protoplast

    Protoplast (from Ancient Greek πρωτόπλαστος (prōtóplastos) 'first-formed'), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. [ 1 ] [ 2 ] Protoplasts can be generated by stripping the cell wall from plant , [ 3 ] bacterial , [ 4 ] [ 5 ] or fungal cells [ 5 ] [ 6 ] by mechanical ...

  3. Protoplasm - Wikipedia

    en.wikipedia.org/wiki/Protoplasm

    In some definitions, it is a general term for the cytoplasm (e.g., Mohl, 1846), [4] but for others, it also includes the nucleoplasm (e.g., Strasburger, 1882). For Sharp (1921), "According to the older usage the extra-nuclear portion of the protoplast [ the entire cell, excluding the cell wall ] was called "protoplasm," but the nucleus also is ...

  4. Lysin - Wikipedia

    en.wikipedia.org/wiki/Lysin

    Double-stranded DNA phage lysins tend to lie within the 25 to 40 kDa range in terms of size. A notable exception is the streptococcal PlyC endolysin, which is 114 kDa. PlyC is not only the biggest and most potent lysin, but also structurally unique since it is composed of two different gene products, PlyCA and PlyCB, with a ratio of eight PlyCB subunits for each PlyCA in its active conformation.

  5. Cell wall - Wikipedia

    en.wikipedia.org/wiki/Cell_wall

    A plant cell wall was first observed and named (simply as a "wall") by Robert Hooke in 1665. [3] However, "the dead excrusion product of the living protoplast" was forgotten, for almost three centuries, being the subject of scientific interest mainly as a resource for industrial processing or in relation to animal or human health.

  6. Lysis - Wikipedia

    en.wikipedia.org/wiki/Lysis

    Penicillin and related β-lactam antibiotics cause the death of bacteria through enzyme-mediated lysis that occurs after the drug causes the bacterium to form a defective cell wall. [3] If the cell wall is completely lost and the penicillin was used on gram-positive bacteria , then the bacterium is referred to as a protoplast , but if ...

  7. Cytoplasmic streaming - Wikipedia

    en.wikipedia.org/wiki/Cytoplasmic_streaming

    Chloroplasts can be seen travelling around the central vacuole of a cell in Rhizomnium punctatum Cytoplasmic streaming in onion epidermal cell. Cytoplasmic streaming, also called protoplasmic streaming and cyclosis, is the flow of the cytoplasm inside the cell, driven by forces from the cytoskeleton. [1]

  8. Active site - Wikipedia

    en.wikipedia.org/wiki/Active_site

    Organisation of enzyme structure and lysozyme example. Binding sites in blue, catalytic site in red and peptidoglycan substrate in black. (In biology and biochemistry, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction.

  9. Coprinopsis cinerea - Wikipedia

    en.wikipedia.org/wiki/Coprinopsis_cinerea

    Coprinopsis cinerea can be transformed with exogenous DNA by transformation when the fungus is a protoplast. It was found that disrupting (knockout or RNAi silencing) ku70 homologue can increase gene targeting via increased homologous recombination. Either protoplasts derived from oidia or vegetative mycelium can be used, however, gene ...