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Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula C 6 H 5 OH. [5] It is a white crystalline solid that is volatile . The molecule consists of a phenyl group ( −C 6 H 5 ) bonded to a hydroxy group ( −OH ).
Phenol (C 6 H 5 OH) is a water-soluble compound consisting of a phenyl group (-C 6 H 5) bonded to a hydroxyl group (-OH). Phenol extraction is a widely used technique for purifying nucleic acid samples from cell lysates. [1] To obtain nucleic acids, the cell must be lysed, and the nucleic acids separated from other cell components.
Phenol: The phenol used for biochemistry comes as a water-saturated solution with Tris buffer, as a Tris-buffered 50% phenol, 50% chloroform solution, or as a Tris-buffered 50% phenol, 48% chloroform, 2% isoamyl alcohol solution (sometimes called "25:24:1"). Phenol is naturally somewhat water-soluble, and gives a fuzzy interface, which is ...
The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols, hydroxamic acids, oximes, and sulfinic acids give positive results as well. [1]
The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987. [ 2 ] TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies , [ 3 ] and Tri-Reagent is the brand name from MRC, [ 4 ] which was founded by Chomczynski.
The Folin–Ciocâlteu reagent (FCR) or Folin's phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE). [1] It is named after Otto Folin, Vintilă Ciocâlteu, and Willey ...
The best-selling drug in the U.S., Acetaminophen, also known as Paracetamol, is a phenol. There are various classification schemes. [15]: 2 A commonly used scheme is based on the number of carbons and was devised by Jeffrey Harborne and Simmonds in 1964 and published in 1980: [15]: 2 [16]
0.5–1% sulfuric acid alcohol for oocysts of isospora, cyclospora. 0.25–0.5% sulfuric acid alcohol for bacterial endospores. Differential staining – glacial acetic acid used, no heat applied, secondary stain is Loeffler's methylene blue. Kinyoun modification (or cold Ziehl–Neelsen technique) is also available.